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Applicable species: mice, rats [12-16,12-17]. Dose range: 1×10^11-1×10^12 vg/mouse; 2×10^11 vg/mouse [12-16,12-17]. Injection volume: mice 5-10 mL/kg (approximately 100-200 μL); rats 10-20 mL/kg (200 μL in literature) [12-16,12-17]. Needle: mouse 25-27 G; rat 23-25 ​​G[12-16]. Speed: Just give it a quick bolus in a few seconds [12-16]. Anesthesia: conscious or light sedation, good immobilization is required [12-16]. Key points of the operation: Fix the head in a low position to move the organs upward, insert a 25 G needle into the left lower abdomen (avoiding the bladder and liver), withdraw intestinal fluid/urine/blood after feeling empty, and then inject again; it can be used to target the liver of neonatal/young mice [12-16]. Coverage: It absorbs into the blood through diaphragm lymph/peritoneal capillaries and then enters the liver through the portal vein. The liver transduction is lower than IV, but the diaphragm, fat, and pancreas transduction is enhanced (AAV6.2, AAV-DJ is obvious), and the rat colon mucosa/submucosa can also be transduced [12-16,12-17]; the peak expression is 2-6 weeks. Limitations: Bioavailability is unstable (affected by abdominal contents, body position, inflammation), and is not suitable for CNS projects that require precise dosage [12-16].