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Applicable species: mice, rats, pigs (skin structure is highly similar to humans) [12-61]. Dose range: The working concentration of mice is 1×10^12 vg/mL, each point is about 2×10^10 vg, and the total dose is 1×10^10-1×10^11 vg/wound [12-61]. Injection volume: 10-20 μL per point, 4-6 points per wound, 80-120 μL in total; 50-100 μL per point in rats [12-61]. Needle: Insulin syringe 29-31 G or Hamilton [12-61]. Anesthesia: Ketamine 80 mg/kg + xylazine 10 mg/kg IP or isoflurane [12-61]. Key points of operation: The tip of the intradermal injection needle is inserted into the skin at an angle of 10-15°, and the wheal can be seen when the needle is injected just after passing through the epidermis; the wound should be injected intradermally at 4-6 points around the wound edge or dripped directly into the wound bed and then covered with Vaseline gauze/hydrogel stent [12-61]. Coverage: Epidermal AAV2/6 can transduce basal keratinocytes (KRT14 promoter), dermal fibroblasts, and wound repair cells; expression peaks in 2-4 weeks [12-61]. Note: The intact stratum corneum of the skin is the main barrier for transdermal penetration, and intradermal injection is limited to a few millimeters around the injection point.