LingXi AAV Genome Optimization Engine
25-40% expression boost through proprietary AI algorithms that perform dual optimization for both target host cells and HEK 293 production cells, instead of relying on generic codon tables.
AAV Design, Optimization, Validation
AAV Genome Optimization & Validation
Design your construct in our platform, validate it in our lab - all in one closed-loop system.
Core Advantages
One coordinated platform from sequence design to validated evidence.
Built for gene therapy teams that need fast, traceable preclinical evidence before committing to clinical-scale manufacturing.
Online Plasmid Design
Customers design plasmids online through Plasmid Combo, visualize constructs in real time, and submit confirmed sequences directly without offline handoffs.
Wet-Dry Closed Loop
Algorithmic optimization, wet-lab validation, data assessment, and optional RUO batch delivery are coordinated in one workflow.
Traceable Data Package
Trace raw sequence inputs, assay reads, analysis outputs, and final reports through every stage of the project with full data traceability.
Lab Evidence
Optimization stays connected to hands-on validation.
Sequence decisions move into bench workflows, assay review, and structured reporting so the optimized design is supported by visible experimental context.
Construct reviewWet-lab validationTraceable report
Workflow
A concise timeline for design, validation, and optional RUO delivery.
Hover or click a step to review the operational detail behind each stage.
Customers log in to the Crossilico platform, use the built-in Plasmid Combo tool to design AAV plasmids online, visualize edits in real time, and submit confirmed constructs directly.
Typical project timing: online design and LingXi optimization begin immediately after submission. Wet-lab validation typically takes 2-4 weeks, with optional RUO batch delivery adding 2-3 weeks.
Technical Scope
Algorithm, production, and analytics are designed as one validation system.
Technical Layers
The service connects the LingXi algorithm layer with production and analytical validation requirements.
| Layer | Core Capability | Output |
|---|---|---|
| Algorithm Layer | LingXi AAV Genome Optimization Engine with dual optimization for target host cells and HEK 293 production cells. | Before-and-after sequence comparison in GenBank / FASTA format. |
| Production Layer | HEK 293 triple-plasmid production system, scalable purification route, and INVIVO / NHP grade production standards. | Production-readiness rationale and optional RUO batch delivery. |
| Analytical Layer | ddPCR titer, SDS-PAGE purity, Refymn mass photometry empty/full ratio, genome integrity, potency, and optional biodistribution analysis. | Technical report, raw data, structured data, and validation interpretation. |
Use Cases
Focused scenarios for early gene therapy development.
Genome Sequence Optimization & Expression Validation
Apply AI dual optimization to AAV genome sequences, validate expression efficiency in the target context, and lock in the best sequence for downstream preclinical development.
Early Pipeline Druggability Assessment
Generate expression, packaging, stability, and characterization evidence to support GO/NO-GO decisions and reduce late-stage development risk.
Deliverables
Standardized outputs for scientific review and data pipelines.
DeliverableFormatDescription
Technical ReportPDF
Figures, conclusions, risk notes, and before-and-after optimization comparison.
Raw DataCSV / Excel
Unprocessed experimental reads with traceable source records.
Structured DataJSON / CSV
Pipeline-ready structured data for downstream computational workflows.
Sequence FilesGenBank / FASTA
Optimized and original sequence records with optimization annotations.
Batch Release ReportPDF
Optional release package for RUO batch delivery, including QC data and testing methods.
Optional Extensions
Add production or analytical modules when the program needs them.
RUO Batch Manufacturing
Validated priority sequences can move into RUO-grade batch production, with optional INVIVO Grade or NHP Grade delivery in 2-3 weeks.
Analytical Testing Add-ons
Add biodistribution research, AAV genome integration risk assessment, or gene editing off-target safety assessment when needed.
FAQ
Common questions.
How is Crossilico different from CDMOs?
CDMOs solve how to manufacture. Crossilico helps decide which sequence to manufacture through AI-driven dual optimization and wet-lab validation.
Do you provide clinical-grade GMP manufacturing?
Crossilico focuses on preclinical work. We provide RUO-grade validated batches and a clinical transition package for GMP partner handoff.
What is the LingXi engine?
LingXi is Crossilico's proprietary AAV genome optimization engine for dual optimization across target host cells and HEK 293 production cells.
How long does a typical project take?
Online design starts immediately. Wet-lab validation typically takes 2-4 weeks, report delivery takes 1 day, and optional RUO batches add 2-3 weeks.
What data formats do you deliver?
Standard delivery includes PDF reports, CSV raw data, and GenBank / FASTA sequences. Custom JSON metadata is available on request.
Start Optimization
Ready to optimize your AAV genome?
Design your construct in Plasmid Combo and get a free computational feasibility assessment. No obligation.