Olfactogenetics
AAV-CMV-DIO-DORs-2A-EGFP-WPREs
Broad-spectrum promoter CMV regulates Cre-dependent tandem non-fusion expression of DORs and EGFP
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, Please consult for more serotypes
DORs
CMV
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
CMV, strong promoter, human cytomegalovirus immediate early enhancer/promoter, will be silenced in some cells, there are two types: 878bp and 1153bp.
Functional Gene Details
DORs system, DORs is composed of Drosophila olfactory receptor 35a (OR35a) and co-receptor Orco; controller: 2-pentanone (flavor agent derived from banana), effector: 2-pentanone odorant receptors (DORs) of Drosophila.
Fluorescent Tag
EGFP
Fluorescent Protein Details
EGFP, green fluorescent protein, has an excitation wavelength of 488nm and an emission wavelength of 507nm. 2A: The 2A sequence is a short peptide sequence. When the ribosome translates it, it will automatically jump away and continue to translate the downstream reading frame, thereby translating the same reading frame into two independent proteins. Used in non-fusion expressions. Commonly used 2A sequences include T2A and P2A.
EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm.
Virus Type
adeno-associated virus
Regulatory Element Details
DIO, Double-floxed inverse Orientation, is an element that cooperates with Cre recombinase to control the expression of the target gene. If there is no Cre recombinase, the target gene will not be expressed, but in the presence of Cre, the target gene can be expressed normally.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.