Optogenetics
rAAV-S5E2-ChrimsonR-P2A-tdTomato
This AAV expresses ChrimsonR-P2A-tdTomato driven by a neuron S5E2 promoter. Details about the source of promoter S5E2 can be found in DOI: 10.1038/s41593-020-0692-9. The report shows that the E2 enhancer has approximately 90% specificity for PV cIN and is confined to the cortex. ChrimosonR is a new type of light-controlled ion channel that can respond to red light around 600nm. On the basis of Chrimson (CnChR1), the point mutation of K176R is made, which speeds up the speed of channel closing, so that at least it can effectively respond to optical signals of 20Hz and below. When used with Chronos, it is possible to control two different neurons with two kinds of fluorescence.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
ChrimsonR
Neuron-specific Promoter
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Delivery Information
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Product Details
Functional Gene Details
ChrimsonR: A new type of light-controlled ion channel that can respond to red light around 600nm was discovered through high-throughput sequencing in the Boyden laboratory. A point mutation of K176R was made on the basis of Chrimson (CnChR1), which speeds up the channel closing speed, making it possible to effectively respond to light signals at least 20Hz and below. When used with Chronos, it is possible to control two different neurons with two fluorophores. The excitation light wavelength is 590nm.
Fluorescent Tag
Tomato
Fluorescent Protein Details
tdTomato: Red fluorescent protein, excitation wavelength 554nm, emission wavelength 581nm.
Expression and Regulation Mode
Others
