rAAV-D1-ChETA-mCherry-WPRE-bGH polyA

Promoter (full value)

D1/D2

D1 receptor promoter(Dopamine receptor promoter, specific expression in D1 receptor–positive neurons)

Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 for mammalian expression. It is maximally excited by 470 nm light. ChETA is a mutant of ChR2, combined with E123T and H134R mutations, has a faster dynamic change. ChETA allows neurons to emit a 200Hz spike when stimulated by a laser, while others ChR2 can only reach 40Hz.

Promoter Details

D1, mouse dopamine D1 receptor gene promoter, 827 bp.

D2, mouse dopamine D2 receptor gene promoter, 1211bp.

D1 receptor promoter(Dopamine receptor promoter, specific expression in D1 receptor–positive neurons)

Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 for mammalian expression. It is maximally excited by 470 nm light. ChETA is a mutant of ChR2, combined with E123T and H134R mutations, has a faster dynamic change. ChETA neurons allows to emit a 200Hz spike when stimulated by a laser, while others ChR2 can only reach 40Hz.

Functional Gene Details

ChETA: There is a point mutation of E123T, which accelerates the closing speed of the ion channel after light withdrawal, making the channel more sensitive to changes in light signals. There is also a mutation of hChR2 (H134R) to increase the inward current. Experiments have shown that ChETA can excite action potentials at a frequency of 200Hz in some neurons, and it is a commonly used high-frequency excitation tool. Compared with ChR2, its maximum excitation wavelength is red-shifted by about 20nm, but it can generally be excited with blue light of about 470nm. The excitation light wavelength is 490nm, suitable for high-frequency light activation.

Fluorescent Tag

mCherry

Fluorescent Protein Details

mCherry: red fluorescent protein, excitation wavelength 587nm, emission wavelength 610nm.

Virus Type

adeno-associated virus

Application

Expressing light-sensitive ion channel proteins, such as ChR2, ChrimsonR, etc., in neurons through rAAV viral vectors can specifically excite neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.

Expression and Regulation Mode

Others