Optogenetics
rAAV-RAM-d2TTA-TRE-hChR2(C128S D156A)-EYFP
Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 for mammalian expression. It is maximally excited by 470 nm light. Stable Step Function Opsin (SSFO) is a double mutant of ChR2 (Asp156Ala and Cys128Ser) with higher light sensitivity than other opsin variants, enabling non-invasive activation of neurons expressing SSFO. The SSFO variant with the additional T159C mutation, known as the SOUL version, has higher light sensitivity in certain brain regions, making it suitable for optogenetic control in deep brain areas.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
hChR2(E123T / T159C)
Inducible Promoter
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Functional Gene Details
hChR2(E123T/T159C): It is called one of the upgraded versions of ChETA in Karl Deisseroth's laboratory. By introducing the point mutation of T159C, the shortcoming of ChETA's weak inward current has been significantly improved. The inward current in the plateau period is close to hChR2(H134R), while also maintaining the rapid closing characteristics of ChETA molecules, giving the channel excellent properties. Based on the E123T mutation, T159C has a significantly better effect on increasing inward current than H134R. The excitation light wavelength is 473nm, suitable for high-frequency light activation.
Fluorescent Tag
EGFP
Fluorescent Protein Details
EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm.
