Calcium Imaging
rAAV-mDlx-DIO-Synaptophysin-jGCaMP7f
jGCaMP7 is a green genetically encoded calcium indicator(GECI) tailored to detect neuronal activity. The jGCaMP7 sensors show substantially better performance than the GCaMP6 sensors in vitro and in vivo, fast kinetics, 3x and 2.5x more sensitive than GCaMP6f and GCaMP6s.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
jGCaMP7
GABAergic interneuron promoter
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Delivery Information
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Product Details
Functional Gene Details
jGCaMP7: By optimizing the Ca2+ affinity of a calcium ion probe while further improving the probe's sensitivity, signal-to-noise ratio, half-life, and dynamic range, Karel Svoboda, Loren Looger, and Vivek from the Howard Hughes Medical Institute Jayaraman and other research groups jointly developed the jGCaMP7 series, including: jGCaMP7s (sensitive), jGCaMP7f (fast kinetics), jGCaMP7b (brighter baseline fluorescence) and jGCaMP7c (high contrast), which are specially used to detect neuronal activity in specific environments. Tracking the dynamics of neuronal subcells is of great significance to the study of neural circuits. However, subcellular structures such as dendrites and axons are small, and jGCaMP6 has low sensitivity and low imaging fidelity. However, jGCaMP7s, jGCaMP7f, and jGCaMP7b have greatly improved the sensitivity under different calcium ion concentrations. jGCaMP7f also has a faster response rate, which is very important for the dynamic detection of neuronal subcells. jGCaMP7 was tested both in vivo and in vitro and performed significantly better than GCaMP6. The sensitivity of jGCaMP7s is more than 5 times that of GCaMP6s, and the speed is faster than GCaMP6s; the sensitivity of jGCaMP7b is 3 times that of GCaMP6s, and the fluorescence brightness is enhanced by 50%; the sensitivity of jGCaMP7c is 2.7 times that of GCaMP6s, and the contrast is enhanced; the reaction rate of jGCaMP7f is 5 times that of GCaMP6f and 3 times that of GCaMP6s. jGCaMP7s more sensitive jGCaMP7f fast kinetics jGCaMP7b high background jGCaMP7c high contrast, low background
Fluorescent Tag
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Fluorescent Protein Details
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
Expression and Regulation Mode
Cre-dependent
