Optogenetics
rAAV-hSyn-hChR2(E123T T159C)-EGFP
Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 for mammalian expression. It is maximally excited by 470 nm light. hCHR2 (E123T/T159C) is a mutated form of ChR2, also known as ChETA (TC), suitable for high-frequency activation with an activation wavelength of 470 nm.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
hChR2(E123T / T159C)
Neuron-specific Promoter
Store at -80°C
Delivery Information
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Product Details
Functional Gene Details
hChR2(E123T/T159C): It is called one of the upgraded versions of ChETA in Karl Deisseroth's laboratory. By introducing the point mutation of T159C, the shortcoming of ChETA's weak inward current has been significantly improved. The inward current in the plateau period is close to hChR2(H134R), while also maintaining the rapid closing characteristics of ChETA molecules, giving the channel excellent properties. Based on the E123T mutation, T159C has a significantly better effect on increasing inward current than H134R. The excitation light wavelength is 473nm, suitable for high-frequency light activation.
Fluorescent Tag
EGFP
Fluorescent Protein Details
EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm.
Expression and Regulation Mode
Others
