rAAV-hSyn-CaMPARI2-WPRE-pA

Promoter Details

hSyn, human synapsin promoter, mature neuron-specific promoter, 448bp.

Functional Gene Details

CaMPARI2.0: EricR Schreiter's team first tested the purified CaMPARI2.0 protein and found that its green and red fluorescence intensities were 60% and 130% higher than the first-generation CaMPARI protein, respectively, and the calcium dissociation rate was also increased several times, making it more sensitive. What is even more exciting is that under calcium-free conditions, the photoconversion rate (PC) of CaMPARI2.0 is significantly lower than that of its predecessor, which means that CaMPARI2.0 will show higher contrast when calcium ion concentrations are different. Using the "super chameleon" CaMPARI2.0 protein, the activities of nerves in a wide range of brain areas in complex behaviors can be studied. CaMPARI2 may have important and widespread application value in the study of brain functional atlas and connectomics. CaMPARI2 CaMPARI2-F391W CaMPARI2-L398T

Application

Measuring neuronal activity using genetically encoded calcium indicators (GECIs) has become a widely used method in neuroscience. Calcium imaging with GECIs is often used to measure neural activity in the complete nervous system, such as detecting the activity of a large number of neuronal cell bodies, or tracking the dynamics of neuronal subcells (such as axons, dendrites, and single synapses). Calcium imaging system is a type of optogenetic technology that uses calcium ion indicators that can sense Ca2+ concentration to detect Ca2+ concentration changes in cells or tissues and convert calcium concentration changes into fluorescent signals, thereby converting cell electrical activity into recordable light signals.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.