rAAV-GFAP(683bp)-CRY2PHR-P2A-CIBN-mCherry-VAMP2-WPREs

Promoter Details

GFAP, human astrocyte-specific promoter, 683bp.

GFAP, human astrocyte-specific promoter, 2207bp.

Functional Gene Details

Opto‑vTrap, a light-induced reversible inhibition system, temporarily blocks the release of neurotransmitters from intracellular to extracellular by trapping synaptic vesicles.

Fluorescent Tag

mCherry

Fluorescent Protein Details

mCherry: red fluorescent protein, excitation wavelength 587nm, emission wavelength 610nm.

Application

Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.