Chemogenetics
rAAV-GfaABC1D-hM3D(Gq)-EGFP
hM3D(Gq) is another modified human-derived G protein-coupled receptor. After activation with clozapine N-oxide (CNO), it activates the Gq signaling pathway to inhibit cells.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
hM3D(Gq)
Glial Cell-specific Promoter
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Delivery Information
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Product Details
Functional Gene Details
hM3D(Gq): It is an artificial receptor modified from the human muscarinic acetylcholine receptor M3 that only responds to CNO and is no longer activated by acetylcholine. In some neurons, after CNO binds hM3D(Gq), it activates the Gq protein-coupled phosphatase PLCβ, causing PIP2 (phosphatidylinositol(4,5)bisphosphate, phosphatidylinositol diphosphate) to be degraded, thereby opening the KCNQ outward potassium ion channel closed by PIP2, so that the cell membrane is depolarized, forming an action potential, that is, activating neurons. In neurons that do not express KCNQ or Gq, the function of hM3D(Gq) may be different.
Fluorescent Tag
EGFP
Fluorescent Protein Details
EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm.
Expression and Regulation Mode
Others
References and DOI
Icariin Mitigates Anxiety-Like Behaviors Induced by Hemorrhage Shock and Resuscitation Via the Inhibition of Astrocytic Activation
