Chemogenetics
rAAV-EF1α-DIO-PSAM4-5HT3 HC-IRES-EGFP
A modified variant of the 5-hydroxytryptamine type 3 receptor (5-HT3R) that incorporates the pharmacological properties of Proline-Specific Adenosine Methyltransferase (PSAM) for chemogenetic control. It is composed of 5-HT3R subunits with PSAM4-specific mutations introduced, enabling specific binding to PSAM ligands and generating ion channel activity. PSAM4-5HT3 retains the cation-selective conductance properties of the native 5-HT3 receptor and depolarizes the cell membrane upon activation by the agonist varenicline, thereby allowing neuronal activation. Reference: PMID 3087
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
PSAM4-5HT3
EF1α
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
Ef1α, a strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where the CMV promoter is silenced, such as stem cells, 1178bp.
EF1α
Fluorescent Tag
EGFP
Fluorescent Protein Details
EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm.
Application
Chemogenetics
Expression and Regulation Mode
Cre-dependent
