rAAV-GfaABC1D-cOpn5-2A-EGFP-WPRE-pA

Promoter Details

GfaABC1D, human small version of astrocyte-specific promoter, 992 bp.

Functional Gene Details

cOpn5: Blue light activated, is a super-sensitive, plug-and-play single-element photoreceptor that can control the Gq signaling pathway of cells at extremely high temporal and spatial resolution, and can be used as an ideal optogenetic tool to study the functions and mechanisms of Gq-coupled receptors that are widely present in cells.

Fluorescent Tag

EGFP

Fluorescent Protein Details

EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm. 2A: The 2A sequence is a short peptide sequence. When the ribosome translates it, it will automatically jump away and continue to translate the downstream reading frame, thereby translating the same reading frame into two independent proteins. Used in non-fusion expressions. Commonly used 2A sequences include T2A and P2A.

EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm.

Application

Expressing light-sensitive ion channel proteins, such as ChR2, ChrimsonR, etc., in neurons through rAAV viral vectors can specifically excite neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.