rAAV-Ef1a-Con Foff-SwiChRca-TS-EYFP-WPRE-hGH polyA

Promoter Details

Ef1α, strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where some CMV promoters are silenced, such as stem cells, 1178bp.

Functional Gene Details

SwiChRca: Step-Waveform Inhibitory ChannelRhodopsin is a chloride channel protein modified by Karl's laboratory based on ChR2. It is very sensitive to blue light, and the channel closing speed is very slow, so short-term light stimulation can continuously activate the channel. Using 632nm red light can speed up the channel closing and end the neuronal inhibition state. The excitation light wavelength is 475nm, and a single light suppression has a long effect.

Fluorescent Tag

EYFP

Fluorescent Protein Details

EYFP: Yellow fluorescent protein, excitation wavelength 513nm, emission wavelength 527nm.

Application

Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Regulatory Element Details

Con Foff

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.