Chemogenetics
rAAV-EF1α-DIO-PSAM4-GlyR-IRES-mCherry
A modified variant of the glycine receptor (GlyR) that incorporates the pharmacological properties of PSAM for chemogenetic regulation. It is composed of GlyR subunits with PSAM4-specific mutations introduced, enabling specific binding to PSAM ligands and generating ion channel activity. PSAM4-GlyR exhibits chloride-selective conductance and hyperpolarizes the cell membrane upon activation by the agonist varenicline, thereby suppressing neuronal activity. Reference: PMID 30872534.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
PSAM4-GlyR
EF1α
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
Ef1α, a strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where the CMV promoter is silenced, such as stem cells, 1178bp.
EF1α
Fluorescent Tag
mCherry
Fluorescent Protein Details
mCherry: red fluorescent protein, excitation wavelength 587nm, emission wavelength 610nm.
Application
Chemogenetics
Expression and Regulation Mode
Cre-dependent
