Optogenetics
rAAV-EF1α-DIO-Jaws-mCherry
Jaws is a red-shifted cruxhalorhodopsin derived from Haloarcula salinarum.It is maximally excited by 632 nm light.Red light causes chloride ions to flow in, which inhibits neuronal activity. Compared with eHpHR3.0 or ArchT, Jaws provides more efficient hyperpolarization through red light gated chloride ion conduction resulting in greater current.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
Jaws
Broad-spectrum Promoter
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Functional Gene Details
Jaws: A chloride ion pump that can be driven by red light and belongs to Halorhodopsin. The maximum excitation wavelength of NpHR is red-shifted by 14 nm. At the same time, the channel current generated by Jaws is about 3 times that of NpHR, making it possible to activate it with 632 nm red light. The advantage of using red light for activation is that red light has stronger penetration into tissues and can operate on a larger area of tissue. It can even suppress Jaws-expressing neurons by giving light outside the skull, which can reduce damage to brain tissue.
Fluorescent Tag
mCherry
Fluorescent Protein Details
mCherry: red fluorescent protein, excitation wavelength 587nm, emission wavelength 610nm.
Expression and Regulation Mode
Cre-dependent
