rAAV-CMV-QuasAr2-WPRE-hGH polyA

Promoter Details

CMV, strong promoter, human cytomegalovirus immediate early enhancer/promoter, will be silenced in some cells, 878, 1153bp.

CMV, strong promoter, human cytomegalovirus immediate early enhancer/promoter, will be silenced in some cells, there are two types: 878bp and 1153bp.

Functional Gene Details

QuasAr2: Excitation light wavelength 640nm.

Application

Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.