Optogenetics
rAAV-CaMKIIa-ReachR-Citrine-WPRE-hGH polyA
Excitatory neuron-specific promoter CaMKIIa regulates the expression of light-activated elements It consists of ChEF/ChIEF, VChR1, VChR2 plus L171I mutation. Can be excited by orange-red light, which provides better membrane transport, higher photocurrent and faster kinetics compared to existing red-shifted ChRs. Transcranial optical activation of neurons in deep brain structures can be achieved without the need to surgically thin the skull, form a transcranial window or implant optical fibers.
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, AAV3, AAV4, AAV11, AAV13, BR1, MG1.AAV2, olig001, 9/retro, Please consult for more serotypes
ReachR ReaChR
CaMKIIa Neuron-specific Promoter
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
CaMKIIa, pyramidal neuron/excitatory neuron promoter, 1293bp.
Functional Gene Details
ReachR: red-activatable ChR, is an optogenetic tool modified by Tsien's laboratory based on vChR1 that can respond to red light with a wavelength of 590 to 630nm. In the range of 10 Hz and below, cortical neurons expressing ReachR can be directly activated by extracranial light administration. However, ReachR can also be excited by blue light.
Fluorescent Tag
Citrine
Fluorescent Protein Details
Citrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm.
Virus Type
adeno-associated virus
Application
Expressing light-sensitive ion channel proteins, such as ChR2, ChrimsonR, etc., in neurons through rAAV viral vectors can specifically excite neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
Expression and Regulation Mode
Others
