Optogenetics
rAAV-CaMKIIa-QuasAr2-mOrange-WPRE-hGH polyA
Excitatory neuron-specific promoter CaMKIIa regulates the expression of photoinhibitory elements
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, Please consult for more serotypes
QuasAr2
CaMKIIa
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
CaMKIIα: Ca2+/calmodulin-dependent protein kinase II, pyramidal neuron/excitatory neuron promoter, 1293bp.
CaMKIIa, pyramidal neuron/excitatory neuron promoter, 1293bp.
Functional Gene Details
QuasAr2: Excitation light wavelength 640nm.
Fluorescent Tag
mOrange
Fluorescent Protein Details
mOrange: Orange fluorescent protein, excitation wavelength 548nm, emission wavelength 562nm.
Virus Type
adeno-associated virus
Application
Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
