Optogenetics
rAAV-CaMKIIα-DIO-eNpHR3.0-EYFP
NpHR is a halorhodopsin or a light-sensitive chloride channel that provides silencing of neuronal activity by hyperpolarization. The addition of the C-terminal trafficking signal from the potassium ion channel Kir2.1 to NpHR gave birth to eNpHR3.0. It offers fast inhibition, and its peak activation falls at 580-680 nm.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
eNpHR3.0
Neuron-specific Promoter
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Functional Gene Details
eNpHR3.0: NpHR is a light-driven chloride ion pump belonging to the Halorhodopsin family. The common version is NpHR3.0, which adds an endoplasmic reticulum anterograde transport signal and a Golgi transport signal to the cell membrane based on NpHR, improving its expression on the cell membrane. It is the most commonly used photoinhibition tool. NpHR is usually driven by yellow light around 570nm and can be used simultaneously with hChR2 (H134R).
Fluorescent Tag
EYFP
Fluorescent Protein Details
EYFP: Yellow fluorescent protein, excitation wavelength 513nm, emission wavelength 527nm.
Expression and Regulation Mode
Cre-dependent
