Optogenetics
PFD-rAAV-mDlx-DIO-Chronos-mCherry-WPRE-hGH polyA
The GABAergic interneuron-specific promoter mDlx regulates the expression of Cre-dependent light-activated elements. Chronos-mCherry is expressed when Cre is present, but Chronos-mCherry is not expressed when Cre is absent.
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, Please consult for more serotypes
Chronos
mDLX
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
mDLX, mouse GABAergic interneuron promoter, 530bp.
Functional Gene Details
Chronos: Through high-throughput sequencing in Boyden's laboratory, it was discovered that high-current light-controlled channels that can respond to blue light can work under low-light conditions, and this power is not enough to activate ChrimsonR, so it can be used in conjunction with ChrimsonR to activate two different types of neurons. Moreover, Chronos has fast channel closing speed and can work at least at 40Hz frequency. The excitation light wavelength is 530nm.
Fluorescent Tag
mCherry
Fluorescent Protein Details
mCherry: red fluorescent protein, excitation wavelength 587nm, emission wavelength 610nm.
Virus Type
adeno-associated virus
Application
Expressing light-sensitive ion channel proteins, such as ChR2, ChrimsonR, etc., in neurons through rAAV viral vectors can specifically excite neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Regulatory Element Details
DIO: Double-floxed inverse Orientation, is an element that cooperates with Cre recombinase to control the expression of the target gene. If there is no Cre recombinase, the target gene will not be expressed, but in the presence of Cre, the target gene can be expressed normally.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
