PFD-rAAV-GFAP-NES-jRCaMP1a-WPRE-hGH polyA

Promoter Details

GFAP, human astrocyte-specific promoter, 2207bp.

Functional Gene Details

jRCaMP1: The excitation light wavelength is red-shifted and can be used together with GCaMP. Contains jRCaMP1a, jRCaMP1b2 species. NES: export nuclear signals, nuclear export signal sequence, only distributed outside the nucleus and located in the cytoplasm.

jRCaMP1: The excitation light wavelength is red-shifted and can be used together with GCaMP. Contains jRCaMP1a, jRCaMP1b2 species.

Application

Measuring neuronal activity using genetically encoded calcium indicators (GECIs) has become a widely used method in neuroscience. Calcium imaging with GECIs is often used to measure neural activity in the complete nervous system, such as detecting the activity of a large number of neuronal cell bodies, or tracking the dynamics of neuronal subcells (such as axons, dendrites, and single synapses). Calcium imaging system is a type of optogenetic technology that uses calcium ion indicators that can sense Ca2+ concentration to detect Ca2+ concentration changes in cells or tissues and convert calcium concentration changes into fluorescent signals, thereby converting cell electrical activity into recordable light signals.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.