Optogenetics
PFD-rAAV-EF1a-fDIO-ChrimsonR-mCherry-WPREs
This AAV expresses ChrimsonR-mCherry driven by a neuron EF1α promoter. ChrimosonR is a new type of light-controlled ion channel that can respond to red light around 600nm. On the basis of Chrimson (CnChR1), the point mutation of K176R is made, which speeds up the speed of channel closing, so that at least it can effectively respond to optical signals of 20Hz and below. When used with Chronos, it is possible to control two different neurons with two kinds of fluorescence.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
ChrimsonR
EF1α Broad-spectrum Promoter
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
Ef1α, strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where some CMV promoters are silenced, such as stem cells, 1178bp.
Functional Gene Details
ChrimsonR: A new type of light-controlled ion channel that can respond to red light around 600nm was discovered through high-throughput sequencing in the Boyden laboratory. A point mutation of K176R was made on the basis of Chrimson (CnChR1), which speeds up the channel closing speed, making it possible to effectively respond to light signals at least 20Hz and below. When used with Chronos, it is possible to control two different neurons with two fluorophores. The excitation light wavelength is 590nm.
Fluorescent Tag
mCherry
Fluorescent Protein Details
mCherry: red fluorescent protein, excitation wavelength 587nm, emission wavelength 610nm.
Application
Expressing light-sensitive ion channel proteins, such as ChR2, ChrimsonR, etc., in neurons through rAAV viral vectors can specifically excite neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Regulatory Element Details
fDIO: It is an element that cooperates with Flp to control the expression of target genes. If there is no Flp recombinase, the target gene will not be expressed. Only in the presence of Flp can the target gene be expressed normally.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
Expression and Regulation Mode
Flp-dependent
References and DOI
Independent optical excitation of distinct neural populations | Nature Methods
