Optogenetics

PFD-rAAV-CamKIIa-hChR2(C128S/D156A/T159C)-P2A-mCherry-WPREs

Optogenetic activation product instructions are for scientific research use only. Please read the instructions carefully before use. rAAV: Recombinant adeno-associated virus, recombinant adeno-associated virus. Using recombinant adeno-associated virus as a vector, it has high safety (rAAV has not yet been found to be pathogenic to humans), low immunogenicity (rarely induces an immune response after infection), has a long expression time, and can infect a variety of cells and tissues. Virus use: rAAV viral vectors are used to express light-sensitive ion channel proteins, such as ChR2, ChrimsonR, etc., in neurons to specifically excite neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior. Promoter: CaMKIIa, pyramidal neuron/excitatory neuron promoter, 1293bp. Functional gene: hChR2(C128S/D156A/T159C): The excitation wavelength is 470nm, no need to implant optical fiber, and light stimulation is applied from outside the skull. Fluorescent protein: mCherry: red fluorescent protein, excitation wavelength 587nm, emission wavelength 610nm. 2A: The 2A sequence is a short peptide sequence. When the ribosome translates it, it will automatically jump away and continue to translate the downstream reading frame, thereby translating the same reading frame into two independent proteins. Used in non-fusion expressions. Commonly used 2A sequences include T2A and P2A. Note: 1. Short-term storage at 4°C; long-term storage at -80°C; 2. It can be stored at -80°C for more than 6 months. Each freeze and thaw will reduce the titer by 10%. Repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use; 3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.