PFD-rAAV-CaMKIIa-Chronos-EGFP-WPRE-hGH polyA

Promoter Details

CaMKIIa, pyramidal neuron/excitatory neuron promoter, 1293bp.

Functional Gene Details

Chronos: Through high-throughput sequencing in Boyden's laboratory, it was discovered that high-current light-controlled channels that can respond to blue light can work under low-light conditions, and this power is not enough to activate ChrimsonR, so it can be used in conjunction with ChrimsonR to activate two different types of neurons. Moreover, Chronos has fast channel closing speed and can work at least at 40Hz frequency. The excitation light wavelength is 530nm.

Fluorescent Tag

EGFP

Fluorescent Protein Details

EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm.

Application

Expressing light-sensitive ion channel proteins, such as ChR2, ChrimsonR, etc., in neurons through rAAV viral vectors can specifically excite neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.