PFD-rAAV-CAG-ArchT-GFP-WPRE-hGH polyA

Promoter Details

CAG, strong promoter, an artificially constructed combined promoter, composed of cytomegalovirus early enhancer and chicken β-actin promoter, 1731bp.

Functional Gene Details

ArchT: Light-driven outward hydrogen ion pump, belonging to Archaerhodopsin. Compared with Arch, although the maximum current it can form is similar to Arch, ArchT is more than three times more sensitive to light and performs well under 1–10 mW/mm2 illumination. Therefore, under the same conditions, the area that can be suppressed is about twice that of Arch. Its maximum activation wavelength is around 570nm, and it can also be activated with light around 532nm. In some neurons, concentrated firing of action potentials occurs after continuous light inhibition.

Fluorescent Tag

GFP

green fluorescent green

Fluorescent Protein Details

GFP: green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm

GFP, green fluorescent protein, excitation wavelength 475 nm, emission wavelength 509 nm.

Virus Type

adeno-associated virus

Adeno-associated virus vector Adeno-associated Virus

Application

Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.

Expression and Regulation Mode

constitutive expression