Optogenetics
pAAV-Ef1a-DIO-ChRmine-EYFP-WPRE
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ChRmine
EF1α
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
Ef1α, a strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where the CMV promoter is silenced, such as stem cells, 1178bp.
Functional Gene Details
ChRmine: The excitation wavelength is 635nm, which can provide larger photocurrent and can also generate wave peaks under low-frequency stimulation, so it can be used for manipulations that require lower heat or deeper brain areas. ChRmine can achieve transcranial light activation of specific neural circuits, including midbrain and brainstem structures, to a staggering depth of 7mm, with millisecond precision, and this behavioral modulation does not require surgical intervention. At the same time, the brain areas manipulated by deep transcranial optogenetics were not damaged by tissue heating or other phototoxic effects.
Fluorescent Tag
EYFP
Fluorescent Protein Details
EYFP: Yellow fluorescent protein, excitation wavelength 513nm, emission wavelength 527nm.
