LV-CaMKIIa-eArch 3.0-EYFP-WPRE

Promoter Details

CaMKIIa, pyramidal neuron/excitatory neuron promoter, 1293bp.

Functional Gene Details

eArch3.0, excitation light wavelength 566nm.

eArch3.0, enhanced light-driven outward hydrogen ion pump, is an engineered variant of Arch (Archaerhodopsin-3). After light activation, hydrogen ions can be pumped from intracellular to extracellular, thereby inducing hyperpolarization of neurons and inhibiting electrical activity. The excitation wavelength is approximately 566 nm. Compared with the original Arch, eArch3.0 achieves higher expression efficiency by optimizing membrane positioning, resulting in stronger photocurrent at the same expression level.

Fluorescent Tag

EYFP

Fluorescent Protein Details

EYFP: Yellow fluorescent protein, excitation wavelength 513nm, emission wavelength 527nm.

Application

Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through lentiviral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.