Chemogenetics
AAV2/9-hEF1a-fDIO-hM3D(Gq)-EGFP-ER2-WPRE-pA
AAV2/2Retro Plus, AAV2/9
hM3D(Gq)
hEF1a
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Delivery Information
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Product Details
Promoter Details
Ef1α, a strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where the CMV promoter is silenced, such as stem cells, 1178bp.
hE F1 a promoter, cloned from the promoter region of human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), is 1.3kb in length, constitutively expressed, and has no cell tissue specificity.
Functional Gene Details
hM3D(Gq): It is an artificial receptor modified from the human muscarinic acetylcholine receptor M3 that only responds to CNO and is no longer activated by acetylcholine. In some neurons, after CNO binds hM3D(Gq), it activates the Gq protein-coupled phosphatase PLCβ, causing PIP2 (phosphatidylinositol(4,5)bisphosphate, phosphatidylinositol diphosphate) to be degraded, thereby opening the KCNQ outward potassium ion channel closed by PIP2, so that the cell membrane is depolarized, forming an action potential, that is, activating neurons. In neurons that do not express KCNQ or Gq, the function of hM3D(Gq) may be different.
Fluorescent Tag
green fluorescent green
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
constitutive expression
