Optogenetics
AAV2/9-hEF1a-CreOn-FlpOff-SwiChRca-TS-EGFP-WPRE-pA
AAV2/9
SwiChRca
hEF1a
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Delivery Information
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Product Details
Promoter Details
Ef1α, a strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where the CMV promoter is silenced, such as stem cells, 1178bp.
Functional Gene Details
SwiChRca: Step-Waveform Inhibitory ChannelRhodopsin is a chloride channel protein modified by Karl's laboratory based on ChR2. It is very sensitive to blue light, and the channel closing speed is very slow, so short-term light stimulation can continuously activate the channel. Using 632nm red light can speed up the channel closing and end the neuronal inhibition state. The excitation light wavelength is 475nm, and a single light suppression has a long effect.
Fluorescent Tag
green fluorescent green
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
constitutive expression
