Optogenetics
rAAV-TRE3g-DIO-hChR2(H134R)-YFP-WPREs
When paired with tTA-expressing viruses or mice, only when there is no dox and cre light-activated elements are expressed; when paired with rtTA-expressing viruses or mice, only when dox and cre exist at the same time, the light-activated elements are expressed.
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, Please consult for more serotypes
hChR2(H134R) TRE tetracycline inducible system
TRE3G
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Additional Categories
Activity-Dependent Expression
Promoter Details
TRE3G, tetracycline-induced expression, 350bp.
Functional Gene Details
hChR2 (H134R): It is the most common form of ChR2. h refers to the coding sequence of the gene that has been optimized for the human codon table. H134R refers to the mutation of histidine at position 134 to arginine. This mutation inhibits the binding of H+ by ChR2 and increases the passage ability of Na+, thereby increasing the overall conductivity of the molecule and thereby increasing the inward current excited by light. Generally, blue light around 470nm is used for excitation.
Fluorescent Tag
YFP
Fluorescent Protein Details
YFP, yellow fluorescent protein, excitation wavelength 513 nm, emission wavelength 527 nm.
Virus Type
adeno-associated virus
Application
Expressing light-sensitive ion channel proteins, such as ChR2, ChrimsonR, etc., in neurons through rAAV viral vectors can specifically excite neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Regulatory Element Details
DIO: Double-floxed inverse Orientation, is an element that cooperates with Cre recombinase to control the expression of the target gene. If there is no Cre recombinase, the target gene will not be expressed, but in the presence of Cre, the target gene can be expressed normally.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
