Optogenetics
rAAV-TRE3G-DIO-eArch3.0-YFP-WPRE-hGHpA
When paired with tTA-expressing viruses or mice, only when there is no dox and cre light-inhibitory elements are expressed; when paired with rtTA-expressing viruses or mice, only when dox and cre exist at the same time, the light-inhibitory elements are expressed. When paired with tTA-expressing viruses or mice, only when there is no dox and cre light-inhibitory elements are expressed; when paired with rtTA-expressing viruses or mice, only when dox and cre are present at the same time, the light-inhibitory elements are expressed
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, Please consult for more serotypes
eArch3.0 TRE tetracycline inducible system
TRE3G
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Additional Categories
Activity-Dependent Expression
Promoter Details
TRE3G, tetracycline-induced expression, 350bp.
Functional Gene Details
eArch3.0, enhanced light-driven outward hydrogen ion pump, is an engineered variant of Arch (Archaerhodopsin-3). After light activation, hydrogen ions can be pumped from intracellular to extracellular, thereby inducing hyperpolarization of neurons and inhibiting electrical activity. The excitation wavelength is approximately 566 nm. Compared with the original Arch, eArch3.0 achieves higher expression efficiency by optimizing membrane positioning, resulting in stronger photocurrent at the same expression level.
Fluorescent Tag
YFP
Fluorescent Protein Details
YFP, yellow fluorescent protein, excitation wavelength 513 nm, emission wavelength 527 nm.
Virus Type
adeno-associated virus
Application
Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Regulatory Element Details
DIO: Double-floxed inverse Orientation, is an element that cooperates with Cre recombinase to control the expression of the target gene. If there is no Cre recombinase, the target gene will not be expressed, but in the presence of Cre, the target gene can be expressed normally.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
