Optogenetics
rAAV-nEF1α-fDIO-Arch-EYFP-WPRE-hGH polyA
The broad-spectrum promoter nEF1α regulates the expression of Flp-dependent light repressive elements. Arch-EYFP is expressed when Flp is present, but Arch-EYFP is not expressed when Flp is absent.
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, Please consult for more serotypes
Arch
nEF1α
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
nEf1α, a smaller version of Ef1α, 212bp.
Functional Gene Details
Arch: Light-driven outward hydrogen ion pump, belonging to Archaerhodopsin. Inhibitory currents of 900pA can be easily reached in neurons, thus inhibiting almost all electrophysiological activities. Although hydrogen ions are not the type of ion used by intrinsic neural electrophysiological activities, the outflow of hydrogen ions can be compensated by the cell's own mechanisms and does not lead to a pH imbalance inside and outside the cell. Arch is generally activated with yellow-green light around 570nm (566nm).
Fluorescent Tag
EYFP
Fluorescent Protein Details
EYFP: Yellow fluorescent protein, excitation wavelength 513nm, emission wavelength 527nm.
Virus Type
adeno-associated virus
Application
Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Regulatory Element Details
fDIO: It is an element that cooperates with Flp to control the expression of target genes. If there is no Flp recombinase, the target gene will not be expressed. Only in the presence of Flp can the target gene be expressed normally.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
