Recombinase
rAAV-miniBEND(mPro723)-Cre-bGHpolyA-mCis700
The optimized mPro723 promoter exhibited high expression activity in endothelial cells, and certain cis-regulatory elements effectively suppressed off-target transcription in non-endothelial cells, among which mCis700 showed the most pronounced effect. Cre is a recombinase derived from bacteriophage P1. This enzyme functions through a topoisomerase I-like mechanism at two DNA recognition sites (LoxP sites).
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
Cre
Tissue-specific Promoter
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Delivery Information
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Product Details
Functional Gene Details
Cre: The most commonly used site-specific DNA recombinase, cloned from bacteriophage P1, consists of 343 amino acids. Cre's action site is a 34bp LoxP sequence with a characteristic structure of ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT. The 13 reverse complementary bases on both sides are Cre's recognition sequences. The middle 8 bases are where recombination occurs. Recombination can only occur if the sequences are the same. N represents a variable base, and different base selections create different LoxP sites. Tissue-specific promoters are used to specifically control the expression of Cre in specific tissues to achieve knockout or transformation of target genes in specific tissue cells. Cre specifically recognizes LoxP sites. If two loxP sites are located on the same DNA strand and in the same direction, Cre recombinase mediates sequence excision between loxPs; if two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence inversion between loxPs; if two loxP sites are located on different DNA strands or chromosomes, Cre recombinase mediates exchange of two DNA strands or chromosomal translocation.
Fluorescent Tag
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Fluorescent Protein Details
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
