RNAi

rAAV-hVGAT-mCherry-5'miR-30a-shRNA(Scramble)-3'miR-30a

ShRNA (short hairpin RNA) is a type of RNA molecule used in gene silencing techniques. It is designed to induce the RNA interference (RNAi) pathway, which leads to the degradation of specific mRNA molecules, thereby reducing or "knocking down" the expression of the target gene. The hVGAT promoter is known as the "Human vesicular GABA transporter promoter," which can specifically drive the expression of exogenous genes in a subset of GABAergic neurons. For the sequence source, please refer to the following link: https://linkinghub.elsevier.com/retrieve/pii/S1044743115300130 .