RNAi

rAAV-GFAP-EGFP-5'miR-30a-shRNA(Scramble)-3'miR-30a

ShRNA (short hairpin RNA) is a type of RNA molecule used in gene silencing techniques. It is designed to induce the RNA interference (RNAi) pathway, which leads to the degradation of specific mRNA molecules, thereby reducing or "knocking down" the expression of the target gene. The GFAP promoter is known as the "Human glial fibrillary acidic protein promoter," which can drive the expression of exogenous genes specifically in astrocytes across various regions of the central nervous system. This promoter is approximately 2200 base pairs in length. For the sequence source, you can refer to the publication with the following PMID: 30962538 .