Helper Viruses
rAAV-Ef1α-DIO-TVA-P2A-NLS-dTomato-WPRE-hGH polyA
Retrovirus avian sarcoma/leukosis virus subtype A receptor (TVA) possesses a 40-amino-acid low-density lipoprotein receptor (LDLR) motif that is sufficient to mediate entry of viruses containing the EnvA envelope protein.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
TVA -
EF1α Broad-spectrum Promoter
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Delivery Information
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Product Details
Promoter Details
Ef1α, a strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where the CMV promoter is silenced, such as stem cells, 1178bp.
Functional Gene Details
hChR2(C128S/D156A/T159C): The excitation wavelength is 470nm, no need to implant optical fiber, and light stimulation is applied from outside the skull.
Fluorescent Tag
dTomato
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Fluorescent Protein Details
dTomato: Orange fluorescent protein, excitation wavelength 554nm, emission wavelength 581nm.
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
Notes
due to policy, the Rabies Virus-related product cannot be sold in the U.S.
Expression and Regulation Mode
Cre-dependent
