Biosensors
rAAV-EF1α-DIO-iGluSnFR3.v857.SGZ
a modified cytosolic fragment of Stargazin (SGZ) increased SNR of optical minis. These variants exhibited narrower optical minis and improved surface trafficking as measured by ΔF/F on glutamate application. GPI and SGZ motifs that increase the SNR of synaptic signals, improve trafficking in cultured neurons, and improve iGluSnFR’s postsynaptic localization in vivo
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
Neurotransmitters Glu
Broad-spectrum Promoter
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Delivery Information
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Product Details
Fluorescent Tag
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Fluorescent Protein Details
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
Expression and Regulation Mode
Cre-dependent
