RNAi

rAAV-(DIO-CaMKIIα)-EGFP-5’miR-30a-shRNA(Scramble)-3’miR-30a

ShRNA (short hairpin RNA) is a type of RNA molecule used in gene silencing techniques. It is designed to induce the RNA interference (RNAi) pathway, which leads to the degradation of specific mRNA molecules, thereby reducing or "knocking down" the expression of the target gene. The CaMKIIα promoter, known as the "Mouse α-calcium-calmodulin dependent kinase II promoter," is capable of driving the expression of exogenous genes specifically in glutamatergic neurons within brain regions such as the forebrain cortex and the hippocampus. The promoter is approximately 1300 base pairs in length. For the sequence source, you can reference the publication identified by the following PMID: 24403157 .