Activity-Dependent Expression
rAAV-CMV-tTA-WPRE-hGH polyA
The tetracycline-inducible system, tTA, is a protein formed by the fusion of TetR and the viral transcription activation domain VP16. Under physiological conditions, tTA actively binds to the TRE, initiating gene expression. However, if doxycycline (Dox) is administered, the binding of Dox to tTA causes dissociation from the TRE, thereby inhibiting gene expression.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
tTA tetracycline inducible system
CMV Broad-spectrum Promoter
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Delivery Information
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Product Details
Promoter Details
CMV, strong promoter, human cytomegalovirus immediate early enhancer/promoter, will be silenced in some cells, there are two types: 878bp and 1153bp.
Fluorescent Tag
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Fluorescent Protein Details
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
