CRISPR
rAAV-CMV-NLS-SaCas9-NLS-3xHA
SpCas9 is derived from Streptococcus pyogenes, a type of bacteria that uses the CRISPR-Cas9 system as part of its immune defense mechanism against viral infections. SpCas9 is widely used in genome editing due to its ability to target and cut specific DNA sequences with high precision.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
SpCas9
Broad-spectrum Promoter
Store at -80°C
Delivery Information
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Product Details
Fluorescent Tag
HA
Fluorescent Protein Details
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
Expression and Regulation Mode
Others
