CRISPR
rAAV-CMV-DIO-SaCas9-3xHA
SpCas9 is derived from Streptococcus pyogenes, a type of bacteria that uses the CRISPR-Cas9 system as part of its immune defense mechanism against viral infections. SpCas9 is widely used in genome editing due to its ability to target and cut specific DNA sequences with high precision.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro, AAV2/9
SpCas9 SaCas9
Broad-spectrum Promoter HCMV(0.6k)
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Additional Categories
CRISPR/Cas - SaCas9
Promoter Details
CMV, strong promoter, human cytomegalovirus immediate early enhancer/promoter, will be silenced in some cells, there are two types: 878bp and 1153bp.
Fluorescent Tag
HA
Fluorescent Protein Details
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
Cre-dependent
constitutive expression
