Optogenetics
rAAV-CaMKIIa-SwiChRca-TS-EYFP-WPRE-hGH polyA
Excitatory neuron-specific promoter CaMKIIa regulates the expression of photoinhibitory elements The C1C2 chimera mutation C128A (SwiChRCA) can slow down the closing of the channel, continuously activate the chloride ion channel by a single blue light, keep the cells in an inhibitory state, and close the chloride ion channel after irradiation with red light.
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, AAV3, AAV4, AAV11, AAV13, BR1, MG1.AAV2, olig001, 9/retro, AAV2/8, Please consult for more serotypes
SwiChRca ChrimsonR
CaMKIIa Neuron-specific Promoter mCaMKIIa
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
CaMKIIa, pyramidal neuron/excitatory neuron promoter, 1293bp.
Functional Gene Details
SwiChRca: Step-Waveform Inhibitory ChannelRhodopsin is a chloride channel protein modified by Karl's laboratory based on ChR2. It is very sensitive to blue light, and the channel closing speed is very slow, so short-term light stimulation can continuously activate the channel. Using 632nm red light can speed up the channel closing and end the neuronal inhibition state. The excitation light wavelength is 475nm, and a single light suppression has a long effect.
ChrimsonR: A new type of light-controlled ion channel that can respond to red light around 600nm was discovered through high-throughput sequencing in the Boyden laboratory. A point mutation of K176R was made on the basis of Chrimson (CnChR1), which speeds up the channel closing speed, making it possible to effectively respond to light signals at least 20Hz and below. When used with Chronos, it is possible to control two different neurons with two fluorophores. The excitation light wavelength is 590nm.
Fluorescent Tag
EYFP
swiqc
yellow fluorescent yellow
Fluorescent Protein Details
EYFP: Yellow fluorescent protein, excitation wavelength 513nm, emission wavelength 527nm.
Virus Type
adeno-associated virus
Adeno-associated virus vector Adeno-associated Virus
Application
Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
Expression and Regulation Mode
Others
specific expression
