Other
rAAV-CAG-pre-mGRASP-mCerulean
The GFP (Green Fluorescent Protein) is split into two components, and each separated GFP component does not possess luminescent functionality. When the two components of GFP are expressed in separate groups of neurons, they automatically bind at synaptic sites to form a complete and functional GFP when synaptic connections exist between the two groups of neurons. At this point, the synaptic structure emits green fluorescence.
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro, AAV2/9
pre-mGRASP
Broad-spectrum Promoter CAG
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Delivery Information
Usually Ships in 7 Business Days；
Product Details
Additional Categories
Split Fluorescent Proteins
Promoter Details
CAG, strong promoter, an artificially constructed combined promoter, composed of cytomegalovirus early enhancer and chicken β-actin promoter, 1731bp.
Fluorescent Tag
-
Cyan fluorescent Cyan
Fluorescent Protein Details
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
constitutive expression
References and DOI
Restoring the Function of Thalamocortical Circuit Through Correcting Thalamic Kv3.2 Channelopathy Normalizes Fear Extinction Impairments in a PTSD Mouse Model
