Optogenetics
rAAV-CAG-DIO-ArchT-EYFP-WPRE-hGH polyA
The broad-spectrum promoter CAG regulates the expression of Cre-dependent light repressive elements. ArchT-EYFP is expressed when Cre is present, but ArchT-EYFP is not expressed when Cre is absent.
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, Please consult for more serotypes
ArchT
CAG
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
CAG, strong promoter, an artificially constructed combined promoter, composed of cytomegalovirus early enhancer and chicken β-actin promoter, 1731bp.
Functional Gene Details
ArchT: Light-driven outward hydrogen ion pump, belonging to Archaerhodopsin. Compared with Arch, although the maximum current it can form is similar to Arch, ArchT is more than three times more sensitive to light and performs well under 1–10 mW/mm2 illumination. Therefore, under the same conditions, the area that can be suppressed is about twice that of Arch. Its maximum activation wavelength is around 570nm (566nm), and it can also be activated with light around 532nm. In some neurons, concentrated firing of action potentials occurs after continuous light inhibition.
ArchT: Light-driven outward hydrogen ion pump, belonging to Archaerhodopsin. Compared with Arch, although the maximum current it can form is similar to Arch, ArchT is more than three times more sensitive to light and performs well under 1–10 mW/mm2 illumination. Therefore, under the same conditions, the area that can be suppressed is about twice that of Arch. Its maximum activation wavelength is around 570nm, and it can also be activated with light around 532nm. In some neurons, concentrated firing of action potentials occurs after continuous light inhibition.
Fluorescent Tag
EYFP
Fluorescent Protein Details
EYFP: Yellow fluorescent protein, excitation wavelength 513nm, emission wavelength 527nm.
Virus Type
adeno-associated virus
Application
Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Regulatory Element Details
DIO: Double-floxed inverse Orientation, is an element that cooperates with Cre recombinase to control the expression of the target gene. If there is no Cre recombinase, the target gene will not be expressed, but in the presence of Cre, the target gene can be expressed normally.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
