Recombinase
rAAV-CAG-CRE-SV40 polyA
Cre is a recombinase derived from bacteriophage P1. This enzyme functions through a topoisomerase I-like mechanism at two DNA recognition sites (LoxP sites).
AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV8, AAV9, retro, AAV11, AAV13, PHP.eB, BR1, B10, MG1.AAV2, olig001, DJ, php.s, 9/retro
Cre -
CAG Broad-spectrum Promoter
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Delivery Information
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Product Details
Promoter Details
CAG, strong promoter, an artificially constructed combined promoter, composed of cytomegalovirus early enhancer and chicken β-actin promoter, 1731bp.
Functional Gene Details
Cre: The most commonly used site-specific DNA recombinase, cloned from bacteriophage P1, consists of 343 amino acids. Cre's action site is a 34bp LoxP sequence with a characteristic structure of ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT. The 13 reverse complementary bases on both sides are Cre's recognition sequences. The middle 8 bases are where recombination occurs. Recombination can only occur if the sequences are the same. N represents a variable base, and different base selections create different LoxP sites. Tissue-specific promoters are used to specifically control the expression of Cre in specific tissues to achieve knockout or transformation of target genes in specific tissue cells. Cre specifically recognizes LoxP sites. If two loxP sites are located on the same DNA strand and in the same direction, Cre recombinase mediates sequence excision between loxPs; if two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence inversion between loxPs; if two loxP sites are located on different DNA strands or chromosomes, Cre recombinase mediates exchange of two DNA strands or chromosomal translocation.
hChR2(C128S/D156A/T159C): The excitation wavelength is 470nm, no need to implant optical fiber, and light stimulation is applied from outside the skull.
Fluorescent Tag
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Fluorescent Protein Details
mCitrine: yellow fluorescent protein, excitation wavelength 516nm, emission wavelength 529nm. Tag protein: HA: The HA tag protein sequence is YPYDVPDYA, which is derived from the hemagglutinin surface epitope of influenza virus. It has 9 amino acids and has little impact on the spatial structure of the foreign target protein. It is easy to construct a tag protein fused to the N-terminus or C-terminus. Easy to detect with Anti-HA antibody detection and ELISA. IRES: internal ribosome entry site, the Chinese name is the internal ribosome entry sequence, which can be inserted downstream of the CDS region on the mRNA to mediate another CDS region that follows it, thus enabling the expression of two proteins on one mRNA.
