Optogenetics
PFD-rAAV-RAM-d2TTA::TRE-ArchT-EGFP-WPRE-pA
Use an optimized activity-dependent promoter to mark active cells; the photorepressive element is expressed under physiological conditions in the Tet-off system, but ArchT is not expressed in the presence of Dox The genetically modified third-generation product has unchanged excitation wavelength, which improves the cell membrane positioning and uniform distribution of light-sensitive tools; compared with NpHR, when the laser is turned off, Arch immediately returns from the channel open state to the closed state.
AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, rh10, DJ, B10, PHP.eB, PHP.S, MaCPNS1/2, retro, anc80l65, AAV3, AAV4, AAV11, AAV13, BR1, MG1.AAV2, olig001, 9/retro, Please consult for more serotypes
ArchT RAM tetracycline induction system
RAM Inducible Promoter
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Additional Categories
Activity-Dependent Expression
Promoter Details
TRE3G, tetracycline-induced expression, 350bp. RAM, activity regulated promoter, 202bp. tTA: tetracycline transcriptional activator, Tet transcriptional activator.
Functional Gene Details
ArchT: Light-driven outward hydrogen ion pump, belonging to Archaerhodopsin. Compared with Arch, although the maximum current it can form is similar to Arch, ArchT is more than three times more sensitive to light and performs well under 1–10 mW/mm2 illumination. Therefore, under the same conditions, the area that can be suppressed is about twice that of Arch. Its maximum activation wavelength is around 570nm (566nm), and it can also be activated with light around 532nm. In some neurons, concentrated firing of action potentials occurs after continuous light inhibition.
ArchT: Light-driven outward hydrogen ion pump, belonging to Archaerhodopsin. Compared with Arch, although the maximum current it can form is similar to Arch, ArchT is more than three times more sensitive to light and performs well under 1–10 mW/mm2 illumination. Therefore, under the same conditions, the area that can be suppressed is about twice that of Arch. Its maximum activation wavelength is around 570nm, and it can also be activated with light around 532nm. In some neurons, concentrated firing of action potentials occurs after continuous light inhibition.
Fluorescent Tag
EGFP
Fluorescent Protein Details
EGFP: Green fluorescent protein, excitation wavelength 488nm, emission wavelength 507nm.
Virus Type
adeno-associated virus
Application
Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.
Notes
1. Short-term storage at 4°C; long-term storage at -80°C
2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use
3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.
Expression and Regulation Mode
Others
