PFD-rAAV-hSyn-Jaws-tdTomato-WPRE-hGH polyA

Promoter Details

hSyn, human synapsin promoter, mature neuron-specific promoter, 448bp.

Functional Gene Details

Jaws: A chloride ion pump that can be driven by red light and belongs to Halorhodopsin. The maximum excitation wavelength of NpHR is red-shifted by 14 nm. At the same time, the channel current generated by Jaws is about 3 times that of NpHR, making it possible to activate it with 632 nm red light. The advantage of using red light for activation is that red light has stronger penetration into tissues and can operate on a larger area of ​​tissue. It can even suppress Jaws-expressing neurons by giving light outside the skull, which can reduce damage to brain tissue.

Fluorescent Tag

tdTomato

Fluorescent Protein Details

tdTomato: Red fluorescent protein, excitation wavelength 554nm, emission wavelength 581nm.

Application

Expressing light-sensitive ion channel proteins, such as eNpHR3.0, Arch, etc., in neurons through rAAV viral vectors can specifically inhibit neurons and explore the effects of light stimulation on neurons, neural circuits and animal behavior.

Notes

1. Short-term storage at 4°C; long-term storage at -80°C

2. It can be stored at -80°C for more than 6 months. Each time it is frozen and thawed, the titer will be reduced by 10%, and repeated freezing and thawing should be avoided; if the virus is stored for more than 6 months, it is recommended to re-measure the virus titer before use

3. According to the experimental needs, the virus can be appropriately diluted and divided into the volume required for each experiment.