Optogenetics
AAV2/9-hSyn-oChIEF-ER2-P2A-mCherry-WPRE-pA
AAV2/9
oChIEF
hSYN(0.5k)
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Delivery Information
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Product Details
Promoter Details
hSyn, human synapsin promoter, mature neuron-specific promoter, 448bp.
The h S yn promoter, also known as the h S yn 1 promoter, is cloned from the promoter region of human synapsin protein 1 (S ynapsin 1). It is about 0.5kb in length and is specifically expressed in neurons.
Functional Gene Details
OChIEF: ChIEF is a fusion of two proteins, ChR1 and ChR2, with Ile mutated to Val at position 170. The characteristic of this protein is that under long-term illumination, the ratio of plateau current to peak current is large, which means that the channel is less inactivated under illumination and is suitable for long-term stimulation. The mutation of Ile accelerates the closing speed of the channel, making ChIEF suitable for high-frequency stimulation. Karl's laboratory has compared the high-frequency stimulation of ChIEF and hChR2(E123A) in pv (small serum protein) neurons, and the effects are equivalent. OChIEF is a codon-optimized version. The excitation light wavelength is 473nm, suitable for high-frequency light activation.
Fluorescent Tag
red fluorescent red
Fluorescent Protein Details
dsRed, red fluorescent protein, excitation wavelength 557nm, emission wavelength 579nm.
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
specific expression
